Technology
Our lab works at the interface of technology development and biological discovery. We believe that integrating emerging technologies in diverse fields allows us to tackle the biggest and most interesting questions in biological science. Together with collaborators at UCSD, across the US, and worldwide, we work to combine the newest and most exciting tools in complimentary ways. We love the idea of “something old and something new” in scientific endeavors, so we also make heavy use of classic approaches in molecular biology, biochemistry, and computational approaches. We are always adopting, innovating, and updating our tools, but listed below are some imaging tools we are currently using heavily:
Simultaneous imaging of individual proteins or pairs of proteins and their local environment in real time
Three-dimensional electron microscopy of freeze-substituted resin-embedded samples
Combining fluorescence microscopy and electron microscopy of frozen samples
Combining high speed imaging, simultaneous photoactivation, and probability analysis to understand protein dynamics